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Azhikina T., Gvozdevsky N., Botvinnik A., Fushan A.,
Shemyakin I., Stepanshina V., Lipin M., Barry C., Sverdlov E. |
A genome-wide sequence-independent comparative analysis of
insertion-deletion polymorphisms in multiple Mycobacterium tuberculosis strains. |
Res Microbiol. 2006 Apr;157(3):282-90. |
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Abstract
We applied an enhanced version of subtractive hybridization for comparative analyses
of indel differences between genomes of several Mycobacterium tuberculosis strains widespread
in Russian regions, and the H37Rv reference strain. A number of differences were detected and
partially analyzed, thus demonstrating the practicality of the approach. A majority of the
insertions found were shared by all Russian strains, except for strain 1540 that revealed
the highest virulence in animal tests. This strain possesses a number of genes absent from
other clinical strains. Two of the differential genes were found to encode putative membrane
proteins and are presumed to affect mycobacterial interaction with the host cell, thus
enhancing virulent properties of the isolate. The method used is of general application,
and enables the elaboration of a catalogue of indel polymorphic genomic differences between
closely related strains. |
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Публикации 2006 г.
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