Abstract Influence of chloramphenicol on electrophysiologic charateristics of Escherichia coli strains
susceptible (K-12 strain) and resistant (pBR-325 strain) to it has been studied. It has been shown
that incubation of susceptible bacteria with chloramphenicol leads to significant change of magnitute
of electrooptic (EO) signal. Significant changes in orientantional spectra of suspensions of susceptible
to chloramphenicol cells incubated with different concentrations of antibiotic were observed only
on first five frequencies of orienting electric field (10 - 1000 kHz). Maximal change of EO signal
occurred at chloramphenicol concentration 35 mg/ml and it didn't depend on the time of antibiotic
exposure. Incubation of resistant strain pBR-325 with chloramphenicol did not lead to change of
EO parameters of cell suspension. Potential for use of electrophysical analytic methods for assessment
of antibacterial activity of chloramphenicol to control effect of antibiotics on microorganisms has
been proposed.
