Efficacy of individual biocides and synergistic combinations
International Biodeterioration & Biodegradation – 2005. – V. 55. – P.
253–259.
Abstract A method for quantitative estimation of the biocidal effect of compounds on microbial cells
has been developed based on colony growth on solid nutrient media. The method allows for
specifying a mode of action and for calculating the most effective ratio of biocides in
synergistic blends. The test organism Serratia marcescens was grown in the presence of copper
sulfate and kathon (2-Noctyl-4-isothiazolin-3-one) on agar medium supplemented with glucose and
peptone. The approach made it possible to achieve respectively 96 and 67% decreases in effective
concentrations of kathon and copper sulfate in the mixture.
