Knirel Y.A, Lindner B., Vinogradov E.,
Shaikhutdinova R.Z., Senchenkova S.N.,
Kocharova N.A., Holst O., Pier G.B., Anisimov A.P. |
|
Abstract
Following a report of variations in the lipopolysaccharide (LPS)
structure of Yersinia pestis at mammalian (37 °C) and flea (25 °C) temperatures, a
number of changes to the LPS structure were observed when
the bacterium was cultivated at a temperature of
winter-hibernating rodents (6 °C). In addition to
one of the known Y. pestis LPS types, LPS of a new type was
isolated from Y. pestis KM218 grown at 6 °C. The
core of the latter differs in: (i) replacement of terminal
galactose with terminal D-glycero-Dmanno- heptose; (ii)
phosphorylation of terminal oct-2-ulosonic acid with
phosphoethanolamine; (iii) a lower content of GlcNAc, and; (iv)
the absence of glycine; lipid A differs in the lack of any
4-amino-4-deoxyarabinose and presumably partial (di)oxygenation
of a fatty acid(s). The data obtained suggest that cold
temperature switches on an alternative mechanism of control
of the synthesis of Y. pestis LPS. |
