Guliy O.I., Ignatov O.V., Shchyogolev S.Yu., Bunin V.D., Ignatov V.V.

Determination of organophosphorus aromatic nitro insecticides by using electric-field cell orientation in microbial suspensions

Analytica Chimica Acta. 2002. - V. 462 – P. 165–177.

Abstract
We examined the effect of the cellular metabolism of the organophosphorus aromatic nitro insecticides metaphos and sumithion on the electro-physical properties (EPPs) of Pseudomonas putida C-11, P. putida BA-11, and Acinetobacter calcoaceticum A-122 suspensions.We used the dependences of cell-suspension absorbance changes induced by electric-field orientation on the orienting-field frequency in the range 10–10,000 kHz. Substantial orientational-spectrum changes, caused by insecticide action, occurred at frequencies of 10–1000 kHz. The plots of electro-optical effect versus insecticide-concentration were linear over the following concentration ranges: 0.5–3.0mM metaphos and 0.5–3.5mM sumithion for P. putida C-11 and BA-11; and 0.5–2.0mM metaphos and 0.5–2.5mM sumithion for A. calcoaceticum A-122. We discuss the possibility of developing a biosensor-method based on the measurement of cell-suspension orientational spectra (OS).

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Публикации 2002 г.