A Selection Method for the Recombinant Clones Bearing the Plague Surface-localized Proteins Encoded by the pFra Plasmid.
Biotechnology in Russia; 2002;(3):65-69.
Abstract The approaches to the selection of recombinant Escherichia coli and Salmonella
minnesota cells containing the genetic determinants of the plasmid pFra from the plague microbe
Yersinia pestis were developed. The products of the plasmid affect the cell surface charge. The
inheritance stability of the recombinant plasmids with the fra operon was determined. The screening
of the gene library of pFra plasmid was carried out, and the method of direct selection of
transformants and superproducers of the antigen of fraction I was suggested.
