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Novikov I.A., Gurov B.N., Shtuchnaia G.V., Fomchenkov V.M., Kholodenko V.P.

[Rapid assay for the assessment of a potential of chemical biocides to microbial destructors of industrial materials]

Prikl Biokhim Mikrobiol. 2001 Jan-Feb;37(1):123-8.

 

Abstract
A colorimetric rapid assay for estimating the biocide potential of various chemicals towards metal biocorrosive and petroleum product degrading microbes was developed based on the reducing potential of live microbial cell. A water-soluble organic redox indicator, blue in the oxidized form and pink in the reduced form, was used as an indicator of the reducing potential of microbial cells. Once added to a suspension of vital microbial cells, it was reduced and changed in color. A good correlation between the results of this assay and viability control was obtained by employing surfactants and heavy metal ions.


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