[Study of the transfer of foreign genes into mussel Mytilus Galloprovincialis Lam. eggs by spermatozoa]
Ontogenez. 2001 Jul-Aug;32(4):309-18.
Abstract The possibility of transferring exogenous DNA into eggs by mussel Mytilus galloprovincialis Lam. sperms
both with the use of certain methods of transfection and without them was studied. The efficacy of egg
fertilization by sperms treated with foreign DNA and the development of larvae at early stages of
embryogenesis were evaluated. Negative effects of the contact between mussel sperms and exogenous DNA
on fertilization and subsequent development were noted. The proportion of developing larvae decreased
with increasing DNA concentration and sperm exposure. Transfer of plasmids pCMVlacZ and pMTbGH into eggs
was observed in group crosses. With the use of PCR, foreign DNA sequences were found in the larvae at the
stage of veliger 48 h after fertilization. An intense signal was recorded after sperm electroporation
in 10% DMSO.
