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Abstract
A method for quantitative evaluation of the effects of biocides is presented. The method was
tested in experiments with Pseudomonas fluorescens grown on various agar nutrient media.
The effective concentrations of biocides that decreased the maximum specific rate of the
colony biomass growth (mu'm) were called S (suppressing) concentrations, and concentrations
that decreased the number of colony-forming units (CFU) were taken as L (sublethal)
concentrations. The efficiency of the reported approach was demonstrated in experiments
with three biocides tested in four nutrient media. It was found that the biocide sensitivity
of Pseudomonas fluorescens varied by a factor of 30, depending on the amount and the type of
the nutrient substrate. |
