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Abstract
Experimental data on transgenic animal production using transfected sperm are reviewed.
Pathways for further improvement of the method for increasing the frequency of transgenic
organisms are proposed: modification of the saccharophosphate skeleton and DNA ends; use
of nucleic exchange enzymes and directed oligonucleotides; use of adhesive sequences (MAR
elements), ori replication, and transposable elements within the transgenes; development
of the embryos selection; the use of gene targeting elements; and the use of various DNA
conformations. The promising use of spermatozoa as a noninvasive system of gene delivery
in transgenesis and prenatal gene therapy are outlined. |
